Ammasi Periasamy | Engineering | Best Researcher Award
University of Virginia | United States
Dr. Periasamy is an internationally renowned scientist recognized for his groundbreaking contributions to the development of advanced optical microscopy techniques for imaging and analyzing single cells, tissues, and living organisms. His pioneering research focuses on designing and developing cutting-edge optical methodologies to study molecular interactions and cellular processes in real time. A major highlight of his recent work is the development of the Fluorescence Lifetime Redox Ratio (FLIRR), an innovative technique used to investigate cellular metabolism and mitochondrial dysfunction, with promising applications in the early detection of prostate cancer and its correlation with PSA levels. Widely regarded as one of the pioneers of fluorescence lifetime imaging microscopy (FLIM), Dr. Periasamy has made significant advances in monitoring calcium oscillations within living cells and developing 2- and 3-color confocal, multiphoton, and FLIM-based Förster resonance energy transfer (FRET) imaging systems for visualizing protein interactions in living specimens. His prolific scholarly output includes over 185 refereed journal publications, numerous book chapters, and proceedings, along with more than 200 invited lectures at national and international platforms. As a respected leader in his field, he has edited three books and serves as the series editor for the “Cellular and Clinical Imaging” book series, encompassing 11 volumes. Dr. Periasamy also plays a key role in the global microscopy community as the chairperson and organizer of the annual SPIE conference on Multiphoton Microscopy in the Biomedical Sciences since 2001 and conducts a highly regarded hands-on training workshop on FLIM, FRET, and metabolic imaging at the University of Virginia each year. In recognition of his outstanding scientific achievements and contributions to optical microscopy, he was elected a Fellow of the SPIE Optical Society in 2012.
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Featured Publications
Alam, S. R., Mahadevan, M. S., & Periasamy, A. (2023). Detecting RNA–Protein Interactions with EGFP-Cy3 FRET by Acceptor Photobleaching. Current Protocols, 3(2), e689.
Norambuena, A., Sun, X., Wallrabe, H., Cao, R., Sun, N., Pardo, E., Shivange, N., Wang, D. B., Post, L. A., Ferris, H. A., Hu, S., Periasamy, A., & Bloom, G. S. (2022). SOD1 mediates lysosome-to-mitochondria communication and its dysregulation by amyloid-β oligomers. Neurobiology of Disease, 169, Article 105737.
Zhang, J., Wallrabe, H., Siller, K., Mbogo, B., Cassidy, T., Alam, S. R., & Periasamy, A. (2025). Measuring metabolic changes in cancer cells using two-photon fluorescence lifetime imaging microscopy and machine-learning analysis. Journal of Biophotonics, 18, e202400426.
Alam, S. R., Wallrabe, H., Christopher, K. G., Siller, K. H., & Periasamy, A. (2022). Characterization of mitochondrial dysfunction due to laser damage by 2-photon FLIM microscopy. Scientific Reports, 12, Article 11938.
Zhou, L., & El-Deiry, W. S. (2009). Multispectral fluorescence imaging. Journal of Nuclear Medicine, 50(10), 1563-1566.